The data provided in this DataSpace consists of sample training data to be used for Fluorescence Reconstruction Microscopy (FRM) testing. We provide a subset of the keratinocyte (10x magnification) dataset used in our paper, in which interested parties may find more complete information about our data collection methods. Matched pairs of phase contrast and fluorescent images are given. The nuclei were stained using Hoechst 33342 and imaged using a standard DAPI filter set.
We provide all the test data and corresponding predictions for our paper, “Practical Fluorescence Reconstruction Microscopy for High-Content Imaging”. Please refer to the Methods section in this paper for experimental details. For each experimental condition, we provide the input transmitted-light images (either phase contrast or DIC), the ground truth fluorescence images, and the output predicted fluorescence images which should reconstruct the ground truth fluorescence images.
Force-driven parallel shear flow in a spatially periodic domain is shown to be linearly unstable
with respect to both the Reynolds number and the domain aspect ratio. This finding is confirmed
by computer simulations, and a simple expression is derived to determine stable flow conditions.
Periodic extensions of Couette and Poiseuille flows are unstable at Reynolds numbers two orders
of magnitude smaller than their aperiodic equivalents because the periodic boundaries impose
fundamentally different constraints. This instability has important implications for designing computational models of nonlinear dynamic processes with periodicity.
Kiefer, Janik; Brunner, Claudia E.; Hansen, Martin O. L.; Hultmark, Marcus
This data set contains data of a NACA 0021 airfoil as it undergoes upward ramp-type pitching motions at high Reynolds numbers and low Mach numbers. The parametric study covers a wide range of chord Reynolds numbers, reduced frequencies and pitching geometries characterized by varying mean angle and angle amplitude. The data were acquired in the High Reynolds number Test Facility at Princeton University, which is a closed-loop wind tunnel that can be pressurized up to 23 MPa and allowed for variation of the chord Reynolds number over a range of 5.0 × 10^5 ≤ Re_c ≤ 5.5 × 10^6. Data were acquired using 32 pressure taps along the surface of the airfoil. The data are the phase-averaged results of 150 individual half-cycles for any given test case.
Ant colonies regulate activity in response to changing conditions without using centralized control. Harvester ant colonies forage in the desert for seeds, and their regulation of foraging manages a tradeoff between spending and obtaining water. Foragers lose water while outside in the dry air, but the colony obtains water by metabolizing the fats in the seeds they eat. Previous work shows that the rate at which an outgoing forager leaves the nest depends on its recent experience of brief antennal contact with returning foragers that carry a seed. We examine how this process can yield foraging rates that are robust to uncertainty and responsive to temperature and humidity across minutes to hour-long timescales. To explore possible mechanisms, we develop a low-dimensional analytical model with a small number of parameters that captures observed foraging behavior. The model uses excitability dynamics to represent response to interactions inside the nest and a random delay distribution to represent foraging time outside the nest. We show how feedback of outgoing foragers returning to the nest stabilizes the incoming and outgoing foraging rates to a common value determined by the ``volatility’’ of available foragers. The model exhibits a critical volatility above which there is sustained foraging at a constant rate and below which there is cessation of foraging. To explain how the foraging rates of colonies adjust to temperature and humidity, we propose a mechanism that relies on foragers modifying their volatility after they leave the nest and get exposed to the environment. Our study highlights the importance of feedback in the regulation of foraging activity and points to modulation of volatility as a key to explaining differences in foraging activity in response to conditions and across colonies. Our results present opportunities for generalization to other contexts and systems with excitability and feedback across multiple timescales.