The data provided in this DataSpace consists of sample training data to be used for Fluorescence Reconstruction Microscopy (FRM) testing. We provide a subset of the keratinocyte (10x magnification) dataset used in our paper, in which interested parties may find more complete information about our data collection methods. Matched pairs of phase contrast and fluorescent images are given. The nuclei were stained using Hoechst 33342 and imaged using a standard DAPI filter set.
The data provided in this DataSpace consists of sample training data to be used for Fluorescence Reconstruction Microscopy (FRM) testing. We provide a subset of the MDCK (20x magnification) dataset used in our paper, in which interested parties may find more complete information about our data collection methods. Matched pairs of DIC and fluorescent images are given. The cells stably expressed E-cadherin:RFP which enabled imaging of junctional fluorescence, while the nuclei were stained using Hoechst 33342 and imaged using a standard DAPI filter set.
We provide all the test data and corresponding predictions for our paper, “Practical Fluorescence Reconstruction Microscopy for High-Content Imaging”. Please refer to the Methods section in this paper for experimental details. For each experimental condition, we provide the input transmitted-light images (either phase contrast or DIC), the ground truth fluorescence images, and the output predicted fluorescence images which should reconstruct the ground truth fluorescence images.
{\rtf1\ansi\ansicpg1252\cocoartf1561\cocoasubrtf600
{\fonttbl\f0\fswiss\fcharset0 Helvetica;}
{\colortbl;\red255\green255\blue255;\red0\green0\blue0;}
{\*\expandedcolortbl;;\cssrgb\c0\c0\c0;}
\margl1440\margr1440\vieww10800\viewh8400\viewkind0
\pard\tx887\tx1775\tx2662\tx3550\tx4438\tx5325\tx6213\tx7101\tx7988\tx8876\tx9764\tx10651\tx11539\tx12427\tx13314\tx14202\tx15090\tx15977\tx16865\tx17753\tx18640\tx19528\tx20416\tx21303\tx22191\tx23079\tx23966\tx24854\tx25742\tx26629\tx27517\tx28405\tx29292\tx30180\tx31067\tx31955\tx32843\tx33730\tx34618\tx35506\tx36393\tx37281\tx38169\tx39056\tx39944\tx40832\tx41719\tx42607\tx43495\tx44382\tx45270\tx46158\tx47045\tx47933\tx48821\tx49708\tx50596\tx51484\tx52371\tx53259\tx54147\tx55034\tx55922\tx56810\tx57697\tx58585\tx59472\tx60360\tx61248\tx62135\tx63023\tx63911\tx64798\tx65686\tx66574\tx67461\tx68349\tx69237\tx70124\tx71012\tx71900\tx72787\tx73675\tx74563\tx75450\tx76338\tx77226\tx78113\tx79001\tx79889\tx80776\tx81664\tx82552\tx83439\tx84327\tx85215\tx86102\tx86990\tx87877\tx88765\slleading20\pardirnatural\partightenfactor0
\f0\fs38 \cf2 The dynamics of the radial envelope of a weak coherent drift wave is approximately governed by a nonlinear Schr\'f6dinger equation, which emerges as a limit of the modified Hasegawa\'97Mima equation. The nonlinear Schr\'f6dinger equation has well-known soliton solutions, and its modulational instability can naturally generate solitary structures. In this paper, we demonstrate that this simple model can adequately describe the formation of solitary zonal structures in the modified Hasegawa\'97Mima equation, but only when the amplitude of the coherent drift wave is relatively small. At larger amplitudes, the modulational instability produces stationary zonal structures instead. Furthermore, we find that incoherent drift waves with beam-like spectra can also be modulationally unstable to the formation of solitary or stationary zonal structures, depending on the beam intensity. Notably, we show that these drift waves can be modeled as quantumlike particles (\'93driftons\'94) within a recently developed phase-space (Wigner\'97Moyal) formulation, which intuitively depicts the solitary zonal structures as quasi-monochromatic drifton condensates. Quantumlike effects, such as diffraction, are essential to these condensates; hence, the latter cannot be described by wave-kinetic models that are based on the ray approximation.\
}
Fractures in geological formations may enable migration of environmentally relevant fluids, as in leakage of CO2 through caprocks in geologic carbon sequestration. We investigated geochemically induced alterations of fracture geometry in Indiana Limestone specimens. Experiments were the first of their kind, with periodic high-resolution imaging using X-ray computed tomography (xCT) scanning while maintaining high pore pressure (100 bar). We studied two CO2-acidified brines having the same pH (3.3) and comparable thermodynamic
disequilibrium but different equilibrated pressures of CO2 (PCO2 values of 12 and 77 bar). High-PCO2 brine has a faster calcite dissolution kinetic rate because of the accelerating effect of carbonic acid. Contrary to expectations, dissolution extents were comparable in the two experiments. However, progressive xCT
images revealed extensive channelization for high PCO2, explained by strong positive feedback between ongoing flow and reaction. The pronounced channel increasingly directed flow to a small region of the fracture, which explains why the overall dissolution was lower than expected. Despite this, flow simulations revealed large increases in permeability in the high-PCO2 experiment. This study shows that the permeability evolution of dissolving fractures will be larger for faster-reacting fluids. The overall mechanism is not because more rock dissolves, as would be commonly assumed, but because of accelerated fracture channelization.
Bertelli, N; Valeo, E.J.; Green, D.L.; Gorelenkova, M.; Phillips, C.K.; Podesta, M.; Lee, J.P.; Wright, J.C.; Jaeger, E.
Abstract:
At the power levels required for significant heating and current drive
in magnetically-confined toroidal plasma, modification of the particle distribution
function from a Maxwellian shape is likely [T. H. Stix, Nucl. Fusion, 15 737
(1975)], with consequent changes in wave propagation and in the location and
amount of absorption. In order to study these effects computationally, both the
finite-Larmor-radius and the high-harmonic fast wave (HHFW), versions of the
full-wave, hot-plasma toroidal simulation code TORIC [M. Brambilla, Plasma Phys.
Control. Fusion 41, 1 (1999) and M. Brambilla, Plasma Phys. Control. Fusion
44, 2423 (2002)], have been extended to allow the prescription of arbitrary velocity
distributions of the form f(v||, v_perp, psi , theta). For hydrogen (H) minority heating of a
deuterium (D) plasma with anisotropic Maxwellian H distributions, the fractional
H absorption varies significantly with changes in parallel temperature but is
essentially independent of perpendicular temperature. On the other hand, for
HHFW regime with anisotropic Maxwellian fast ion distribution, the fractional
beam ion absorption varies mainly with changes in the perpendicular temperature.
The evaluation of the wave-field and power absorption, through the full wave
solver, with the ion distribution function provided by either aMonte-Carlo particle
and Fokker-Planck codes is also examined for Alcator C-Mod and NSTX plasmas.
Non-Maxwellian effects generally tends to increase the absorption with respect to
the equivalent Maxwellian distribution.
Menard, J.E.; Brown, T.; El-Guebaly, L.; Boyer, M.; Canik, J.; Colling, B.; Raman, R.; Wang, Z.; Zhai, Y.; Buxton, P.; Covele, B.; D'Angelo, C.; Davis, A.; Gerhardt, S.; Gryaznevich, M.; Harb, M.; Hender, T.C.; Kaye, S.; Kingham, D.; Kotschenreuther, M.; Mahajan, S.; Maingi, R.; Marriott, E.; Meier, E.T.; Mynsberge, L.; Neumeyer, C.; Ono, M.; Park, J.-K.; Sabbagh, S.A.; Soukhanovskii, V.; Valanju, P.; Woolley, R.
Abstract:
A Fusion Nuclear Science Facility (FNSF) could play an important role in the development of fusion energy by providing the nuclear environment needed to develop fusion materials and components. The spherical torus/tokamak (ST) is a leading candidate for an FNSF due to its potentially high neutron wall loading and modular configuration. A key consideration for the choice of FNSF configuration is the range of achievable missions as a function of device size. Possible missions include: providing high neutron wall loading and fluence, demonstrating tritium self-sufficiency, and demonstrating electrical self-sufficiency. All of these missions must also be compatible with a viable divertor, first-wall, and blanket solution. ST-FNSF configurations have been developed simultaneously incorporating for the first time: (1) a blanket system capable of tritium breeding ratio TBR approximately 1, (2) a poloidal field coil set supporting high elongation and triangularity for a range of internal inductance and normalized beta values consistent with NSTX/NSTX-U previous/planned operation, (3) a long-legged divertor analogous to the MAST-U divertor which substantially reduces projected peak divertor heat-flux and has all outboard poloidal field coils outside the vacuum chamber and superconducting to reduce power consumption, and (4) a vertical maintenance scheme in which blanket structures and the centerstack can be removed independently. Progress in these ST-FNSF missions vs. configuration studies including dependence on plasma major radius R0 for a range 1m to 2.2m are described. In particular, it is found the threshold major radius for TBR = 1 is R0 greater than or equal to 1.7m, and a smaller R0=1m ST device has TBR approximately 0.9 which is below unity but substantially reduces T consumption relative to not breeding. Calculations of neutral beam heating and current drive for non-inductive ramp-up and sustainment are described. An A=2, R0=3m device incorporating high-temperature superconductor toroidal field coil magnets capable of high neutron fluence and both tritium and electrical self-sufficiency is also presented following systematic aspect ratio studies.
