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21. Attentional Modulation of Brain Responses to Primary Appetitive and Aversive Stimuli

Author(s):
Cara L. Buck; Jonathan D. Cohen; Field, Brent; Daniel Kahneman; Samuel M. McClure; Leigh E. Nystrom
Abstract:
Studies of subjective well-being have conventionally relied upon self-report, which directs subjects’ attention to their emotional experiences. This method presumes that attention itself does not influence emotional processes, which could bias sampling. We tested whether attention influences experienced utility (the moment-by-moment experience of pleasure) by using functional magnetic resonance imaging (fMRI) to measure the activity of brain systems thought to represent hedonic value while manipulating attentional load. Subjects received appetitive or aversive solutions orally while alternatively executing a low or high attentional load task. Brain regions associated with hedonic processing, including the ventral striatum, showed a response to both juice and quinine. This response decreased during the high-load task relative to the low-load task. Thus, attentional allocation may influence experienced utility by modulating (either directly or indirectly) the activity of brain mechanisms thought to represent hedonic value.
Type:
Dataset, Software, and text
Issue Date:
11 February 2015

22. Blob Structure and Motion in the Edge and SOL of NSTX

Author(s):
Zweben, S.J.; J.R. Myra; W.M. Davis; D.A. D'Ippolito; T.K. Gray; S.M. Kaye; B.P. LeBlanc; R.J. Maqueda; D.A. Russell; D.P. Stotler
Abstract:
Blob analysis dataset
Type:
Dataset
Issue Date:
January 2016

23. Blob wakes in NSTX

Author(s):
Zweben SJ, Myra JR, Diallo A, Russell DA, Scotti F, Stotler DP
Abstract:
Transient small-scale structures were identified in the wake of blobs movingpoloidally through the SOL of high-powered H-mode plasmas in NSTX, using the gaspuff imaging (GPI) diagnostic. These blob wakes had a poloidal wavelength in therange 3.5 cm, which is significantly smaller than the average blob scale of~12 cm, and the wakes had a poloidal velocity of 1.5 km/sec in theelectron diamagnetic direction, which is opposite to the blob poloidal velocity inthese shots. These wakes were radially localized 0-4 cm outside the separatrix andoccurred within ~50 microsec after the passage of a blob through the GPI field of view.The clearest wakes were seen when the GPI viewing angle was well aligned with thelocal B field line, as expected for such small-scale structures given the diagnosticgeometry. A plausible theoretical interpretation of the wakes is discussed: theobserved wakes share some features of drift waves and/or drift-Alfven waves whichcould be excited
Type:
Dataset
Issue Date:
July 2019

24. Blob-hole correlation model for edge turbulence and comparisons with NSTX GPI data

Author(s):
Myra, J.R.; Zweben, S.J.; Russell, D.A.
Abstract:
Gas puff imaging (GPI) observations made in NSTX [Zweben S J, et al., 2017 Phys. Plasmas 24 102509] have revealed two-point spatial correlations of edge and scrape-off layer turbulence in the plane perpendicular to the magnetic field. A common feature is the occurrence of dipole-like patterns with significant regions of negative correlation. In this paper, we explore the possibility that these dipole patterns may be due to blob-hole pairs. Statistical methods are applied to determine the two-point spatial correlation that results from a model of blob-hole pair formation. It is shown that the model produces dipole correlation patterns that are qualitatively similar to the GPI data in several respects. Effects of the reference location (confined surfaces or scrape-off layer), a superimposed random background, hole velocity and lifetime, and background sheared flows are explored and discussed with respect to experimental observations. Additional analysis of the experimental GPI dataset is performed to further test this blob-hole correlation model. A time delay two-point spatial correlation study did not reveal inward propagation of the negative correlation structures that were postulated to correspond to holes in the data nor did it suggest that the negative correlation structures are due to neutral shadowing. However, tracing of the highest and lowest values (extrema) of the normalized GPI fluctuations shows strong evidence for mean inward propagation of minima and outward propagation of maxima, in qualitative agreement with theoretical expectations. Other properties of the experimentally observed extrema are discussed.
Type:
Dataset
Issue Date:
July 2018

25. CKavity Library: Next-Generation Sequencing

Abstract:
Protein sequence space is vast; nature uses only an infinitesimal fraction of possible sequences to sustain life. Are there solutions to biological problems other than those provided by nature? Can we create artificial proteins that sustain life? To investigate this question, the Hecht lab has created combinatorial collections, or libraries, of novel sequences with no homology to those found in living organisms. These libraries were subjected to screens and selections, leading to the identification of sequences with roles in catalysis, modulating gene regulation, and metal homeostasis. However, the resulting functional proteins formed dynamic rather than well-ordered structures. This impeded structural characterization and made it difficult to ascertain a mechanism of action. To address this, Christina Karas's thesis work focuses on developing a new model of libraries based on the de novo protein S-824, a four-helix bundle with a very stable three-dimensional structure. The first part of this research focused on mutagenesis of S-824 and characterization of the resulting proteins, revealing that this scaffold tolerates amino acid substitutions, including buried polar residues and the removal of hydrophobic side chains to create a putative cavity. Distinct from previous libraries, Karas targeted variability to a specific region of the protein, seeking to create a cavity and potential active site. The second part of this work details the design and creation of a library encoding 1.7 x 10^6 unique proteins, assembled from degenerate oligonucleotides. The third and fourth parts of this work cover the screening effort for a range of activities, both in vitro and in vivo. I found that this collection binds heme readily, leading to abundant peroxidase activity. Hits for lipase and phosphatase activity were also detected. This work details the development of a new strategy for creating de novo sequences geared toward function rather than structure.
Type:
Dataset

26. CLEVR-Matrices

Author(s):
Mondal, Shanka Subhra; Webb, Taylor; Cohen, Jonathan
Abstract:
A dataset of Raven’s Progressive Matrices (RPM)-like problems using realistically rendered 3D shapes, based on source code from CLEVR (a popular visual-question-answering dataset) (Johnson, J., Hariharan, B., Van Der Maaten, L., Fei-Fei, L., Lawrence Zitnick, C., & Girshick, R. (2017). Clevr: A diagnostic dataset for compositional language and elementary visual reasoning. In Proceedings of the IEEE conference on computer vision and pattern recognition (pp. 2901-2910)).
Type:
Dataset
Issue Date:
1 March 2023

28. Calibrationless rotating Lorentz-force flowmeters for low flow rate applications

Author(s):
Hvasta, M. G.; Dudt, D.; Fisher, A. E.; Kolemen, E.
Abstract:
A 'weighted magnetic bearing' has been developed to improve the performance of rotating Lorentz-force flowmeters (RLFFs). Experiments have shown that the new bearing reduces frictional losses within a double-sided, disc-style RLFF to negligible levels. Operating such an RLFF under 'frictionless' conditions provides two major benefits. First, the steady-state velocity of the RLFF magnets matches the average velocity of the flowing liquid at low flow rates. This enables an RLFF to make accurate volumetric flow measurements without any calibration or prior knowledge of the fluid properties. Second, due to minimized frictional losses, an RLFF is able to measure low flow rates that cannot be detected when conventional, high-friction bearings are used. This paper provides a brief background on RLFFs, gives a detailed description of weighted magnetic bearings, and compares experimental RLFF data to measurements taken with a commercially available flowmeter.
Type:
Dataset
Issue Date:
29 May 2018

29. Climate Impacts from Large Volcanic Eruptions in a High-resolution Climate Model: the Importance of Forcing Structure

Author(s):
Yang, Wenchang; Vecchi, Gabriel; Fueglistaler, Stephan; Horowitz, Larry; Luet, David; Muñoz, Ángel; Paynter, David; Underwood, Seth
Abstract:
Explosive volcanic eruptions have large climate impacts, and can serve as observable tests of the climatic response to radiative forcing. Using a high resolution climate model, we contrast the climate responses to Pinatubo, with symmetric forcing, and those to Santa Maria and Agung, which had meridionally asymmetric forcing. Although Pinatubo had larger global-mean forcing, asymmetric forcing strongly shifts the latitude of tropical rainfall features, leading to larger local precipitation/TC changes. For example, North Atlantic TC activity over is enhanced/reduced by SH-forcing (Agung)/NH-forcing (Santa Maria), but changes little in response to the Pinatubo forcing. Moreover, the transient climate sensitivity estimated from the response to Santa Maria is 20% larger than that from Pinatubo or Agung. This spread in climatic impacts of volcanoes needs to be considered when evaluating the role of volcanoes in global and regional climate, and serves to contextualize the well-observed response to Pinatubo.
Type:
Dataset
Issue Date:
2019

30. Code and data from "Comparative genomic analysis reveals varying levels of mammalian adaptation to coronavirus infections"

Author(s):
King, Sean
Abstract:
Severe acute respiratory coronavirus 2 (SARS-CoV-2), the causative agent of COVID-19, is of zoonotic origin. Evolutionary analyses assessing whether coronaviruses similar to SARS-CoV-2 infected ancestral species of modern-day animal hosts could be useful in identifying additional reservoirs of potentially dangerous coronaviruses. We reasoned that if a clade of species has been repeatedly exposed to a virus, then their proteins relevant for viral entry may exhibit adaptations that affect host susceptibility or response. We perform comparative analyses across the mammalian phylogeny of angiotensin-converting enzyme 2 (ACE2), the cellular receptor for SARS-CoV-2, in order to uncover evidence for selection acting at its binding interface with the SARS-CoV-2 spike protein. We uncover that in rodents there is evidence for adaptive amino acid substitutions at positions comprising the ACE2-spike interaction interface, whereas the variation within ACE2 proteins in primates and some other mammalian clades is not consistent with evolutionary adaptations. We also analyze aminopeptidase N (APN), the receptor for the human coronavirus 229E, a virus that causes the common cold, and find evidence for adaptation in primates. Altogether, our results suggest that the rodent and primate lineages may have had ancient exposures to viruses similar to SARS-CoV-2 and HCoV-229E, respectively. Included in this repository are the instructions and corresponding code required to build the dataset and run the analysis in the manuscript.
Type:
Dataset
Issue Date:
28 September 2021