Fluorescence Reconstruction Microscopy Data: MDCK (20x) DIC to Nuclei (DAPI) and E-Cadherin (RFP)

LaChance, Julienne; Cohen, Daniel
Issue date: 24 February 2020
Cite as:
LaChance, Julienne & Cohen, Daniel. (2020). Fluorescence Reconstruction Microscopy Data: MDCK (20x) DIC to Nuclei (DAPI) and E-Cadherin (RFP) [Data set]. Princeton University. https://doi.org/10.34770/jgvp-c928
@electronic{lachance_julienne_2020,
  author      = {LaChance, Julienne and
                Cohen, Daniel},
  title       = {{Fluorescence Reconstruction Microscopy D
                ata: MDCK (20x) DIC to Nuclei (DAPI) and
                 E-Cadherin (RFP)}},
  publisher   = {{Princeton University}},
  year        = 2020,
  url         = {https://doi.org/10.34770/jgvp-c928}
}
Abstract:

The data provided in this DataSpace consists of sample training data to be used for Fluorescence Reconstruction Microscopy (FRM) testing. We provide a subset of the MDCK (20x magnification) dataset used in our paper, in which interested parties may find more complete information about our data collection methods. Matched pairs of DIC and fluorescent images are given. The cells stably expressed E-cadherin:RFP which enabled imaging of junctional fluorescence, while the nuclei were stained using Hoechst 33342 and imaged using a standard DAPI filter set.

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# Filename Description Filesize
1 Training_Data_MDCK_20x.zip 237 MB
2 FRM_Dataset_README.txt 926 Bytes