Data For “Development of a dual channel detection system for pan-genotypic simultaneous quantification of hepatitis B and delta viruses"

Ploss, Alexander; Liu, Yongzhen
Issue date: 2024
Cite as:
Ploss, Alexander & Liu, Yongzhen. (2024). Data For “Development of a dual channel detection system for pan-genotypic simultaneous quantification of hepatitis B and delta viruses" [Data set]. Princeton University.
  author      = {Ploss, Alexander and
                Liu, Yongzhen},
  title       = {{Data For “Development of a dual channel
                detection system for pan-genotypic simul
                taneous quantification of hepatitis B an
                d delta viruses"}},
  publisher   = {{Princeton University}},
  year        = 2024,
  url         = {}

Hepatitis B virus (HBV) infection remains a major public health problem and, in associated co-infection with hepatitis delta virus (HDV), causes the most severe viral hepatitis and accelerated liver disease progression. As a defective satellite RNA virus, HDV can only propagate in the presence of HBV infection, which makes HBV DNA and HDV RNA the standard biomarkers for monitoring the virological response upon antiviral therapy, in co-infected patients. Although assays have been described to quantify these viral nucleic acids in circulation independently, a method for monitoring both viruses simultaneously is not available, thus hampering characterization of their complex dynamic interactions. Here, we describe the development of a dual fluorescence channel detection system for pan-genotypic, simultaneous quantification of HBV DNA and HDV RNA through a one-step quantitative PCR. The sensitivity for both HBV and HDV is about 10 copies per microliter without significant interference between these two detection targets. This assay provides reliable detection for HBV and HDV basic research in vitro and in human liver chimeric mice. Preclinical validation of this system on serum samples from patient on or off antiviral therapy also illustrates a promising application that is rapid and cost-effective in monitoring HBV and HDV viral loads simultaneously.

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# Filename Description Filesize
1 20240429-DATASET_Development of a dual channel detection ... README file 41 KB
2 Fig 1B.csv 352 Bytes
3 Fig 1C.csv 362 Bytes
4 Fig 1D.csv 464 Bytes
5 Fig 1F.csv 506 Bytes
6 Fig 1G.csv 823 Bytes
7 Fig 1H.csv 732 Bytes
8 Fig 1I.csv 435 Bytes
9 Fig 2C.csv 126 Bytes
10 Fig 2D.csv 155 Bytes
11 Fig 2E.csv 445 Bytes
12 Fig 3A.csv 952 Bytes
13 Fig 3B.csv 634 Bytes
14 Fig 3C.csv 536 Bytes
15 Fig 3D.csv 443 Bytes
16 Fig 3E western blot images-repeat experiment.jpg 548 KB
17 Fig 3E western blot images.jpg 622 KB
18 Fig 3E.csv 335 Bytes
19 Fig 4A.csv 81 Bytes
20 Fig 4B and C-orginal images.jpg 1.86 MB
21 Fig 4D.csv 548 Bytes
22 Fig 4E.csv 392 Bytes
23 Fig 4F.csv 233 Bytes
24 Fig 5C.csv 540 Bytes
25 Fig 5D.csv 471 Bytes
26 Fig 5E.csv 482 Bytes
27 Fig 5F.csv 310 Bytes
28 Fig 6A.csv 366 Bytes
29 Fig 6B.csv 370 Bytes
30 Fig 6D.csv 521 Bytes
31 Fig 6E.csv 332 Bytes
32 Supplementary Fig 1D.csv 194 Bytes
33 Supplementary Fig 2-HBV DNA.csv 156 Bytes
34 Supplementary Fig 2-HBV DNA.jpg 60.3 KB
35 Supplementary Fig 2-HDV RNA.csv 157 Bytes
36 Supplementary Fig 2-HDV RNA.jpg 66.7 KB
37 Supplementary Fig 4A.csv 154 Bytes
38 Supplementary Fig 4B.csv 943 Bytes
39 Supplementary Fig 4C.csv 400 Bytes