Distinct cytoskeletal proteins define zones of enhanced cell wall synthesis in Helicobacter pylori

Taylor, Jenny A.; Bratton, Benjamin P.; Sichel, Sophie R.; Blair, Kris M.; Jacobs, Holly M.; DeMeester, Kristen E.; Kuru, Erkin; Gray, Joe; Biboy, Jacob; VanNieuwenhze, Michael S.; Vollmer, Waldemar; Grimes, Catherine L.; Shaevitz, Joshua W.; Salama, Nina R.
Issue date: April 2019
Cite as:
Taylor, Jenny A., Bratton, Benjamin P., Sichel, Sophie R., Blair, Kris M., Jacobs, Holly M., DeMeester, Kristen E., Kuru, Erkin, Gray, Joe, Biboy, Jacob, VanNieuwenhze, Michael S., Vollmer, Waldemar, Grimes, Catherine L., Shaevitz, Joshua W., & Salama, Nina R. (2020). Distinct cytoskeletal proteins define zones of enhanced cell wall synthesis in Helicobacter pylori [Data set]. https://doi.org/10.34770/r2dz-ys12
@electronic{taylor_jenny_a_2020,
  author      = {Taylor, Jenny A. and
                Bratton, Benjamin P. and
                Sichel, Sophie R. and
                Blair, Kris M. and
                Jacobs, Holly M. and
                DeMeester, Kristen E. and
                Kuru, Erkin and
                Gray, Joe and
                Biboy, Jacob and
                VanNieuwenhze, Michael S. and
                Vollmer, Waldemar and
                Grimes, Catherine L. and
                Shaevitz, Joshua W. and
                Salama, Nina R.},
  title       = {{Distinct cytoskeletal proteins define zo
                nes of enhanced cell wall synthesis in H
                elicobacter pylori}},
  year        = 2020,
  url         = {https://doi.org/10.34770/r2dz-ys12}
}
Abstract:

Helical cell shape is necessary for efficient stomach colonization by Helicobacter pylori, but the molecular mechanisms for generating helical shape remain unclear. We show that the helical centerline pitch and radius of wild-type H. pylori cells dictate surface curvatures of considerably higher positive and negative Gaussian curvatures than those present in straight- or curved-rod bacteria. Quantitative 3D microscopy analysis of short pulses with either N-acetylmuramic acid or D-alanine metabolic probes showed that cell wall growth is enhanced at both sidewall curvature extremes. Immunofluorescence revealed MreB is most abundant at negative Gaussian curvature, while the bactofilin CcmA is most abundant at positive Gaussian curvature. Strains expressing CcmA variants with altered polymerization properties lose helical shape and associated positive Gaussian curvatures. We thus propose a model where CcmA and MreB promote PG synthesis at positive and negative Gaussian curvatures, respectively, and that this patterning is one mechanism necessary for maintaining helical shape.

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Description:

This dataset includes structured illumination fluorescence microscopy images (SIM) and their associated cell shape reconstructions, phase contrast micrographs, and transmission electron micrographs. See the README.txt for detailed description of the strains and conditions represented in each data file.

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# Filename Description Filesize
1 README.txt 28.2 KB
2 Fig11_JTH6_D-Ala-alk_1.tgz 1.29 GB
3 Fig11_JTH6_D-Ala-alk_2.tgz 2.76 GB
4 Fig11_JTH6_D-Ala-alk_3.tgz 3.02 GB
5 Fig11_JTH6_D-Ala-alk_4.tgz 3.07 GB
6 Fig11_JTH6_D-Ala-alk_5.tgz 1.18 GB
7 Fig11_JTH6_D-Ala-alk_6.tgz 2.58 GB
8 Fig11_JTH6_D-Ala-alk_7.tgz 1.82 GB
9 Fig11_JTH6_MurNAc-alk_1.tgz 1.11 GB
10 Fig11_JTH6_MurNAc-alk_2.tgz 3 GB
11 Fig11_JTH6_MurNAc-alk_3.tgz 3.03 GB
12 Fig11_JTH6_MurNAc-alk_4.tgz 1.53 GB
13 Fig11_JTH6_MurNAc-alk_5.tgz 2.36 GB
14 Fig11_JTH6_anti-MreB_1.tgz 1.7 GB
15 Fig11_JTH6_anti-MreB_2.tgz 3.26 GB
16 Fig11_JTH6_anti-MreB_3.tgz 3.18 GB
17 Fig11_JTH6_anti-MreB_4.tgz 2.13 GB
18 Fig11_JTH6_anti-MreB_5.tgz Re-uploaded due to checksum mismatch. 2.59 GB
19 Fig11_JTH6_anti-MreB_Preimmune_1.tgz 1.78 GB
20 Fig11_JTH6_anti-MreB_Preimmune_2.tgz 2.04 GB
21 Fig11_JTH6_anti-MreB_Preimmune_3.tgz 2.91 GB
22 Fig11_JTH6_anti-MreB_Preimmune_4.tgz 2.99 GB
23 Fig11_JTH6_anti-MreB_Preimmune_5.tgz 2.84 GB
24 Fig11_JTH6_anti-MreB_Preimmune_6.tgz 2.18 GB
25 Fig11_JTH6_nolabel_1.tgz 1.22 GB
26 Fig11_JTH6_nolabel_2.tgz 1.88 GB
27 Fig11_JTH6_nolabel_3.tgz 1.95 GB
28 Fig11_JTH6_nolabel_4.tgz 2.8 GB
29 Fig11_JTH6_nolabel_5.tgz 2.76 GB
30 Fig11_JTH6_nolabel_6.tgz 2.73 GB
31 Fig11_JTH6_nolabel_7.tgz 2.44 GB
32 Fig2_LSH100_1.tgz 3.32 GB
33 Fig2_LSH100_2.tgz 2.46 GB
34 Fig2_LSH100_3.tgz 1.99 GB
35 Fig2_LSH100_4.tgz 1.89 GB
36 Fig2_LSH100_5.tgz 1.96 GB
37 Fig2_LSH100_6.tgz 1.61 GB
38 Fig2_deltaCsd2_1.tgz 2.52 GB
39 Fig2_deltaCsd2_2.tgz 2.97 GB
40 Fig2_deltaCsd2_3.tgz 3.19 GB
41 Fig2_deltaCsd2_4.tgz 3.36 GB
42 Fig2_deltaCsd2_5.tgz 3.35 GB
43 Fig2_deltaCsd2_6.tgz 2.77 GB
44 Fig2_deltaCsd6_1.tgz 3.17 GB
45 Fig2_deltaCsd6_2.tgz 2.73 GB
46 Fig2_deltaCsd6_3.tgz 2.68 GB
47 Fig2_deltaCsd6_4.tgz 2.57 GB
48 Fig2_deltaCsd6_5.tgz 2.51 GB
49 Fig2_deltaCsd6_6.tgz 1.91 GB
50 Fig2_deltaCsd6_7.tgz 1.34 GB
51 Fig5and6_LSH108KU_D-Ala-alk_18min_rep1_1.tgz 1.98 GB
52 Fig5and6_LSH108KU_D-Ala-alk_18min_rep1_2.tgz 2.66 GB
53 Fig5and6_LSH108KU_D-Ala-alk_18min_rep2_1.tgz 967 MB
54 Fig5and6_LSH108KU_D-Ala-alk_18min_rep2_2.tgz 2.4 GB
55 Fig5and6_LSH108KU_D-Ala-alk_18min_rep2_3.tgz 1.13 GB
56 Fig5and6_LSH108KU_D-Ala-alk_18min_rep3_1.tgz 1.3 GB
57 Fig5and6_LSH108KU_D-Ala-alk_18min_rep3_2.tgz 2.47 GB
58 Fig5and6_LSH108KU_MurNAc-alk_18min_rep1_1.tgz 858 MB
59 Fig5and6_LSH108KU_MurNAc-alk_18min_rep1_2.tgz Re-uploaded due to tar error. 2.72 GB
60 Fig5and6_LSH108KU_MurNAc-alk_18min_rep1_3.tgz 2.72 GB
61 Fig5and6_LSH108KU_MurNAc-alk_18min_rep2_1.tgz 1.16 GB
62 Fig5and6_LSH108KU_MurNAc-alk_18min_rep2_2.tgz 2.7 GB
63 Fig5and6_LSH108KU_MurNAc-alk_18min_rep2_3.tgz 2.7 GB
64 Fig5and6_LSH108KU_MurNAc-alk_18min_rep3_1.tgz 480 MB
65 Fig5and6_LSH108KU_MurNAc-alk_18min_rep3_2.tgz 1.92 GB
66 Fig5and6_LSH108KU_nolabel_rep1_1.tgz 789 MB
67 Fig5and6_LSH108KU_nolabel_rep1_2.tgz 3.35 GB
68 Fig5and6_LSH108KU_nolabel_rep2_1.tgz 1.88 GB
69 Fig5and6_LSH108KU_nolabel_rep2_2.tgz 3.29 GB
70 Fig5and6_LSH108KU_nolabel_rep3_1.tgz 1.64 GB
71 Fig5and6_LSH108KU_nolabel_rep3_2.tgz 2.61 GB
72 Fig5and6_LSH108KU_nolabel_rep3_3.tgz 1.32 GB
73 Fig7_LSH108KU_anti-MreB-Preimmune_rep1_1.tgz 765 MB
74 Fig7_LSH108KU_anti-MreB-Preimmune_rep1_2.tgz 2.73 GB
75 Fig7_LSH108KU_anti-MreB-Preimmune_rep2_1.tgz 1.61 GB
76 Fig7_LSH108KU_anti-MreB-Preimmune_rep2_2.tgz 2.73 GB
77 Fig7_LSH108KU_anti-MreB-Preimmune_rep3_1.tgz 1.14 GB
78 Fig7_LSH108KU_anti-MreB-Preimmune_rep3_2.tgz 2.51 GB
79 Fig7_LSH108KU_anti-MreB-Preimmune_rep3_3.tgz 1.87 GB
80 Fig7_LSH108KU_anti-MreB_rep1_1.tgz 1.29 GB
81 Fig7_LSH108KU_anti-MreB_rep1_2.tgz 2.8 GB
82 Fig7_LSH108KU_anti-MreB_rep2_1.tgz 2.28 GB
83 Fig7_LSH108KU_anti-MreB_rep2_2.tgz 2.63 GB
84 Fig7_LSH108KU_anti-MreB_rep3_1.tgz 989 MB
85 Fig7_LSH108KU_anti-MreB_rep3_2.tgz 2.48 GB
86 Fig7_LSH108KU_anti-MreB_rep3_3.tgz 1.77 GB
87 Fig7and8_2xMreBSequences_TEM_Celltool_1.tgz 233 MB
88 Fig9and10_LSH100_CcmApreimmune_1.tgz 1.85 GB
89 Fig9and10_LSH100_CcmApreimmune_2.tgz 2.66 GB
90 Fig9and10_LSH100_CcmApreimmune_3.tgz 2.73 GB
91 Fig9and10_LSH100_CcmApreimmune_4.tgz 3.07 GB
92 Fig9and10_LSH100_CcmApreimmune_5.tgz 3.08 GB
93 Fig9and10_LSH100_antiCcmA_1.tgz 1.56 GB
94 Fig9and10_LSH100_antiCcmA_2.tgz 2.64 GB
95 Fig9and10_LSH100_antiCcmA_3.tgz 2.11 GB
96 Fig9and10_LSH100_antiCcmA_4.tgz 2.37 GB
97 Fig9and10_LSH108KU-CcmA-FLAG_rep1_1.tgz 520 MB
98 Fig9and10_LSH108KU-CcmA-FLAG_rep1_2.tgz 3 GB
99 Fig9and10_LSH108KU-CcmA-FLAG_rep2_1.tgz 505 MB
100 Fig9and10_LSH108KU-CcmA-FLAG_rep2_2.tgz 2.92 GB
101 Fig9and10_LSH108KU-CcmA-FLAG_rep3_1.tgz 507 MB
102 Fig9and10_LSH108KU-CcmA-FLAG_rep3_2.tgz 3.02 GB
103 Fig9and10_LSH108KU_anti-FLAG_rep1_1.tgz 1.06 GB
104 Fig9and10_LSH108KU_anti-FLAG_rep1_2.tgz 2.93 GB
105 Fig9and10_LSH108KU_anti-FLAG_rep2_1.tgz 1.12 GB
106 Fig9and10_LSH108KU_anti-FLAG_rep2_2.tgz 2.99 GB
107 Fig9and10_LSH108KU_anti-FLAG_rep3_1.tgz 1.08 GB
108 Fig9and10_LSH108KU_anti-FLAG_rep3_2.tgz 2.86 GB
109 Fig9and10_SSH1_CcmApreimmune_1.tgz 2 GB
110 Fig9and10_SSH1_CcmApreimmune_2.tgz 2.77 GB
111 Fig9and10_SSH1_CcmApreimmune_3.tgz 2.78 GB
112 Fig9and10_SSH1_CcmApreimmune_4.tgz Re-uploaded due to checksum mismatch. 2.93 GB
113 Fig9and10_SSH1_CcmApreimmune_5.tgz 3.06 GB
114 Fig9and10_SSH1_CcmApreimmune_6.tgz 3.02 GB
115 Fig9and10_SSH1_CcmApreimmune_7.tgz 3.01 GB
116 Fig9and10_SSH1_antiCcmA_1.tgz 1.2 GB
117 Fig9and10_SSH1_antiCcmA_2.tgz 2.78 GB
118 Fig9and10_SSH1_antiCcmA_3.tgz 2.26 GB
119 Fig9and10_SSH1_antiCcmA_4.tgz 2.28 GB
120 Fig9and10_SSH1_antiCcmA_5.tgz 2.27 GB
121 Fig9and10_SSH1_antiCcmA_6.tgz 2.45 GB
122 Fig9and10_SSH1_antiCcmA_7.tgz 2.62 GB
123 Fig9and10_SSH2_CcmApreimmune_1.tgz Re-uploaded due to tar error. 1.26 GB
124 Fig9and10_SSH2_CcmApreimmune_2.tgz 3.09 GB
125 Fig9and10_SSH2_CcmApreimmune_3.tgz 3.05 GB
126 Fig9and10_SSH2_CcmApreimmune_4.tgz 3.14 GB
127 Fig9and10_SSH2_CcmApreimmune_5.tgz 2.87 GB
128 Fig9and10_SSH2_CcmApreimmune_6.tgz 1020 MB
129 Fig9and10_SSH2_antiCcmA_1.tgz 1.27 GB
130 Fig9and10_SSH2_antiCcmA_2.tgz 2.95 GB
131 Fig9and10_SSH2_antiCcmA_3.tgz 2.8 GB
132 Fig9and10_SSH2_antiCcmA_4.tgz 2.77 GB
133 Fig9and10_SSH2_antiCcmA_5.tgz 2.72 GB
134 Fig9and10_SSH2_antiCcmA_6.tgz 1.55 GB
135 PrincetonUniversity-shae-cellshape-public-1.2.1.tgz Re-uploaded due to tar error. 83.2 MB