Distinct cytoskeletal proteins define zones of enhanced cell wall synthesis in Helicobacter pylori

Taylor, Jenny A.; Bratton, Benjamin P.; Sichel, Sophie R.; Blair, Kris M.; Jacobs, Holly M.; DeMeester, Kristen E.; Kuru, Erkin; Gray, Joe; Biboy, Jacob; VanNieuwenhze, Michael S.; Vollmer, Waldemar; Grimes, Catherine L.; Shaevitz, Joshua W.; Salama, Nina R.
Issue date: April 2019
Cite as:
Taylor, Jenny A., Bratton, Benjamin P., Sichel, Sophie R., Blair, Kris M., Jacobs, Holly M., DeMeester, Kristen E., Kuru, Erkin, Gray, Joe, Biboy, Jacob, VanNieuwenhze, Michael S., Vollmer, Waldemar, Grimes, Catherine L., Shaevitz, Joshua W., & Salama, Nina R. (2020). Distinct cytoskeletal proteins define zones of enhanced cell wall synthesis in Helicobacter pylori [Data set]. https://doi.org/10.34770/r2dz-ys12
@electronic{taylor_jenny_a_2020,
  author      = {Taylor, Jenny A. and
                Bratton, Benjamin P. and
                Sichel, Sophie R. and
                Blair, Kris M. and
                Jacobs, Holly M. and
                DeMeester, Kristen E. and
                Kuru, Erkin and
                Gray, Joe and
                Biboy, Jacob and
                VanNieuwenhze, Michael S. and
                Vollmer, Waldemar and
                Grimes, Catherine L. and
                Shaevitz, Joshua W. and
                Salama, Nina R.},
  title       = {{Distinct cytoskeletal proteins define zo
                nes of enhanced cell wall synthesis in H
                elicobacter pylori}},
  year        = 2020,
  url         = {https://doi.org/10.34770/r2dz-ys12}
}
Abstract:

Helical cell shape is necessary for efficient stomach colonization by Helicobacter pylori, but the molecular mechanisms for generating helical shape remain unclear. We show that the helical centerline pitch and radius of wild-type H. pylori cells dictate surface curvatures of considerably higher positive and negative Gaussian curvatures than those present in straight- or curved-rod bacteria. Quantitative 3D microscopy analysis of short pulses with either N-acetylmuramic acid or D-alanine metabolic probes showed that cell wall growth is enhanced at both sidewall curvature extremes. Immunofluorescence revealed MreB is most abundant at negative Gaussian curvature, while the bactofilin CcmA is most abundant at positive Gaussian curvature. Strains expressing CcmA variants with altered polymerization properties lose helical shape and associated positive Gaussian curvatures. We thus propose a model where CcmA and MreB promote PG synthesis at positive and negative Gaussian curvatures, respectively, and that this patterning is one mechanism necessary for maintaining helical shape.

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Description:

This dataset includes structured illumination fluorescence microscopy images (SIM) and their associated cell shape reconstructions, phase contrast micrographs, and transmission electron micrographs. See the README.txt for detailed description of the strains and conditions represented in each data file.

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# Filename Description Filesize
1 README.txt 28.2 KB
2 Fig2_LSH100_1.tgz 3.32 GB
3 Fig2_LSH100_2.tgz 2.46 GB
4 Fig2_LSH100_3.tgz 1.99 GB
5 Fig2_LSH100_4.tgz 1.89 GB
6 Fig2_LSH100_5.tgz 1.96 GB
7 Fig2_LSH100_6.tgz 1.61 GB
8 Fig2_deltaCsd2_1.tgz 2.52 GB
9 Fig2_deltaCsd2_2.tgz 2.97 GB
10 Fig2_deltaCsd2_3.tgz 3.19 GB
11 Fig2_deltaCsd2_4.tgz 3.36 GB
12 Fig2_deltaCsd2_5.tgz 3.35 GB
13 Fig2_deltaCsd2_6.tgz 2.77 GB
14 Fig2_deltaCsd6_1.tgz 3.17 GB
15 Fig2_deltaCsd6_2.tgz 2.73 GB
16 Fig2_deltaCsd6_3.tgz 2.68 GB
17 Fig2_deltaCsd6_4.tgz 2.57 GB
18 Fig2_deltaCsd6_5.tgz 2.51 GB
19 Fig2_deltaCsd6_6.tgz 1.91 GB
20 Fig2_deltaCsd6_7.tgz 1.34 GB
21 Fig5and6_LSH108KU_D-Ala-alk_18min_rep1_1.tgz 1.98 GB
22 Fig5and6_LSH108KU_D-Ala-alk_18min_rep1_2.tgz 2.66 GB
23 Fig5and6_LSH108KU_D-Ala-alk_18min_rep2_1.tgz 967 MB
24 Fig5and6_LSH108KU_D-Ala-alk_18min_rep2_2.tgz 2.4 GB
25 Fig5and6_LSH108KU_D-Ala-alk_18min_rep2_3.tgz 1.13 GB
26 Fig5and6_LSH108KU_D-Ala-alk_18min_rep3_1.tgz 1.3 GB
27 Fig5and6_LSH108KU_D-Ala-alk_18min_rep3_2.tgz 2.47 GB
28 Fig5and6_LSH108KU_MurNAc-alk_18min_rep1_1.tgz 858 MB
29 Fig5and6_LSH108KU_MurNAc-alk_18min_rep1_3.tgz 2.72 GB
30 Fig5and6_LSH108KU_MurNAc-alk_18min_rep2_1.tgz 1.16 GB
31 Fig5and6_LSH108KU_MurNAc-alk_18min_rep2_2.tgz 2.7 GB
32 Fig5and6_LSH108KU_MurNAc-alk_18min_rep2_3.tgz 2.7 GB
33 Fig5and6_LSH108KU_MurNAc-alk_18min_rep3_1.tgz 480 MB
34 Fig5and6_LSH108KU_MurNAc-alk_18min_rep3_2.tgz 1.92 GB
35 Fig5and6_LSH108KU_nolabel_rep1_1.tgz 789 MB
36 Fig5and6_LSH108KU_nolabel_rep1_2.tgz 3.35 GB
37 Fig5and6_LSH108KU_nolabel_rep2_1.tgz 1.88 GB
38 Fig5and6_LSH108KU_nolabel_rep2_2.tgz 3.29 GB
39 Fig5and6_LSH108KU_nolabel_rep3_1.tgz 1.64 GB
40 Fig5and6_LSH108KU_nolabel_rep3_2.tgz 2.61 GB
41 Fig5and6_LSH108KU_nolabel_rep3_3.tgz 1.32 GB
42 Fig7and8_2xMreBSequences_TEM_Celltool_1.tgz 233 MB
43 Fig7_LSH108KU_anti-MreB-Preimmune_rep1_1.tgz 765 MB
44 Fig7_LSH108KU_anti-MreB-Preimmune_rep1_2.tgz 2.73 GB
45 Fig7_LSH108KU_anti-MreB-Preimmune_rep2_1.tgz 1.61 GB
46 Fig7_LSH108KU_anti-MreB-Preimmune_rep2_2.tgz 2.73 GB
47 Fig7_LSH108KU_anti-MreB-Preimmune_rep3_1.tgz 1.14 GB
48 Fig7_LSH108KU_anti-MreB-Preimmune_rep3_2.tgz 2.51 GB
49 Fig7_LSH108KU_anti-MreB-Preimmune_rep3_3.tgz 1.87 GB
50 Fig7_LSH108KU_anti-MreB_rep1_1.tgz 1.29 GB
51 Fig7_LSH108KU_anti-MreB_rep1_2.tgz 2.8 GB
52 Fig7_LSH108KU_anti-MreB_rep2_1.tgz 2.28 GB
53 Fig7_LSH108KU_anti-MreB_rep2_2.tgz 2.63 GB
54 Fig7_LSH108KU_anti-MreB_rep3_1.tgz 989 MB
55 Fig7_LSH108KU_anti-MreB_rep3_2.tgz 2.48 GB
56 Fig7_LSH108KU_anti-MreB_rep3_3.tgz 1.77 GB
57 Fig9and10_LSH100_antiCcmA_1.tgz 1.56 GB
58 Fig9and10_LSH100_antiCcmA_2.tgz 2.64 GB
59 Fig9and10_LSH100_antiCcmA_3.tgz 2.11 GB
60 Fig9and10_LSH100_antiCcmA_4.tgz 2.37 GB
61 Fig9and10_LSH100_CcmApreimmune_1.tgz 1.85 GB
62 Fig9and10_LSH100_CcmApreimmune_2.tgz 2.66 GB
63 Fig9and10_LSH100_CcmApreimmune_3.tgz 2.73 GB
64 Fig9and10_LSH100_CcmApreimmune_4.tgz 3.07 GB
65 Fig9and10_LSH100_CcmApreimmune_5.tgz 3.08 GB
66 Fig9and10_LSH108KU_anti-FLAG_rep1_1.tgz 1.06 GB
67 Fig9and10_LSH108KU_anti-FLAG_rep1_2.tgz 2.93 GB
68 Fig9and10_LSH108KU_anti-FLAG_rep2_1.tgz 1.12 GB
69 Fig9and10_LSH108KU_anti-FLAG_rep2_2.tgz 2.99 GB
70 Fig9and10_LSH108KU_anti-FLAG_rep3_1.tgz 1.08 GB
71 Fig9and10_LSH108KU_anti-FLAG_rep3_2.tgz 2.86 GB
72 Fig9and10_LSH108KU-CcmA-FLAG_rep1_1.tgz 520 MB
73 Fig9and10_LSH108KU-CcmA-FLAG_rep1_2.tgz 3 GB
74 Fig9and10_LSH108KU-CcmA-FLAG_rep2_1.tgz 505 MB
75 Fig9and10_LSH108KU-CcmA-FLAG_rep2_2.tgz 2.92 GB
76 Fig9and10_LSH108KU-CcmA-FLAG_rep3_1.tgz 507 MB
77 Fig9and10_LSH108KU-CcmA-FLAG_rep3_2.tgz 3.02 GB
78 Fig9and10_SSH1_antiCcmA_1.tgz 1.2 GB
79 Fig9and10_SSH1_antiCcmA_2.tgz 2.78 GB
80 Fig9and10_SSH1_antiCcmA_3.tgz 2.26 GB
81 Fig9and10_SSH1_antiCcmA_4.tgz 2.28 GB
82 Fig9and10_SSH1_antiCcmA_5.tgz 2.27 GB
83 Fig9and10_SSH1_antiCcmA_6.tgz 2.45 GB
84 Fig9and10_SSH1_antiCcmA_7.tgz 2.62 GB
85 Fig9and10_SSH1_CcmApreimmune_1.tgz 2 GB
86 Fig9and10_SSH1_CcmApreimmune_2.tgz 2.77 GB
87 Fig9and10_SSH1_CcmApreimmune_3.tgz 2.78 GB
88 Fig9and10_SSH1_CcmApreimmune_5.tgz 3.06 GB
89 Fig9and10_SSH1_CcmApreimmune_6.tgz 3.02 GB
90 Fig9and10_SSH1_CcmApreimmune_7.tgz 3.01 GB
91 Fig9and10_SSH2_antiCcmA_1.tgz 1.27 GB
92 Fig9and10_SSH2_antiCcmA_2.tgz 2.95 GB
93 Fig9and10_SSH2_antiCcmA_3.tgz 2.8 GB
94 Fig9and10_SSH2_antiCcmA_4.tgz 2.77 GB
95 Fig9and10_SSH2_antiCcmA_5.tgz 2.72 GB
96 Fig9and10_SSH2_antiCcmA_6.tgz 1.55 GB
97 Fig9and10_SSH2_CcmApreimmune_2.tgz 3.09 GB
98 Fig9and10_SSH2_CcmApreimmune_3.tgz 3.05 GB
99 Fig9and10_SSH2_CcmApreimmune_4.tgz 3.14 GB
100 Fig9and10_SSH2_CcmApreimmune_5.tgz 2.87 GB
101 Fig9and10_SSH2_CcmApreimmune_6.tgz 1020 MB
102 Fig11_JTH6_anti-MreB_1.tgz 1.7 GB
103 Fig11_JTH6_anti-MreB_2.tgz 3.26 GB
104 Fig11_JTH6_anti-MreB_3.tgz 3.18 GB
105 Fig11_JTH6_anti-MreB_4.tgz 2.13 GB
106 Fig11_JTH6_anti-MreB_Preimmune_1.tgz 1.78 GB
107 Fig11_JTH6_anti-MreB_Preimmune_2.tgz 2.04 GB
108 Fig11_JTH6_anti-MreB_Preimmune_3.tgz 2.91 GB
109 Fig11_JTH6_anti-MreB_Preimmune_4.tgz 2.99 GB
110 Fig11_JTH6_anti-MreB_Preimmune_5.tgz 2.84 GB
111 Fig11_JTH6_anti-MreB_Preimmune_6.tgz 2.18 GB
112 Fig11_JTH6_D-Ala-alk_1.tgz 1.29 GB
113 Fig11_JTH6_D-Ala-alk_2.tgz 2.76 GB
114 Fig11_JTH6_D-Ala-alk_3.tgz 3.02 GB
115 Fig11_JTH6_D-Ala-alk_4.tgz 3.07 GB
116 Fig11_JTH6_D-Ala-alk_5.tgz 1.18 GB
117 Fig11_JTH6_D-Ala-alk_6.tgz 2.58 GB
118 Fig11_JTH6_D-Ala-alk_7.tgz 1.82 GB
119 Fig11_JTH6_MurNAc-alk_1.tgz 1.11 GB
120 Fig11_JTH6_MurNAc-alk_2.tgz 3 GB
121 Fig11_JTH6_MurNAc-alk_3.tgz 3.03 GB
122 Fig11_JTH6_MurNAc-alk_4.tgz 1.53 GB
123 Fig11_JTH6_MurNAc-alk_5.tgz 2.36 GB
124 Fig11_JTH6_nolabel_1.tgz 1.22 GB
125 Fig11_JTH6_nolabel_2.tgz 1.88 GB
126 Fig11_JTH6_nolabel_3.tgz 1.95 GB
127 Fig11_JTH6_nolabel_4.tgz 2.8 GB
128 Fig11_JTH6_nolabel_5.tgz 2.76 GB
129 Fig11_JTH6_nolabel_6.tgz 2.73 GB
130 Fig11_JTH6_nolabel_7.tgz 2.44 GB
131 Fig9and10_SSH1_CcmApreimmune_4.tgz Re-uploaded due to checksum mismatch. 2.93 GB
132 Fig11_JTH6_anti-MreB_5.tgz Re-uploaded due to checksum mismatch. 2.59 GB
133 PrincetonUniversity-shae-cellshape-public-1.2.1.tgz Re-uploaded due to tar error. 83.2 MB
134 Fig5and6_LSH108KU_MurNAc-alk_18min_rep1_2.tgz Re-uploaded due to tar error. 2.72 GB
135 Fig9and10_SSH2_CcmApreimmune_1.tgz Re-uploaded due to tar error. 1.26 GB