Download from Globus
Issue date: 2024
Maya, Stephanie, Carver, Sebastian, O’Connell, Aoife, Zen, Anna, Gertje, Hans, Seneca, Kathleen, Nahass, Ronald, Crossland, Nicholas, Ploss, Alexander, & Liu, Yongzhen. (2024). Data For “Development of a dual channel detection system for pan-genotypic simultaneous quantification of hepatitis B and delta viruses" [Data set]. Princeton University. https://doi.org/10.34770/s4zx-ab39
-
Description: Hepatitis B virus (HBV) infection remains a major public health problem and, in associated co-infection with hepatitis delta virus (HDV), causes the most severe viral hepatitis and accelerated liver disease progression. As a defective satellite RNA virus, HDV can only propagate in the presence of HBV infection, which makes HBV DNA and HDV RNA the standard biomarkers for monitoring the virological response upon antiviral therapy, in co-infected patients. Although assays have been described to quantify these viral nucleic acids in circulation independently, a method for monitoring both viruses simultaneously is not available, thus hampering characterization of their complex dynamic interactions. Here, we describe the development of a dual fluorescence channel detection system for pan-genotypic, simultaneous quantification of HBV DNA and HDV RNA through a one-step quantitative PCR. The sensitivity for both HBV and HDV is about 10 copies per microliter without significant interference between these two detection targets. This assay provides reliable detection for HBV and HDV basic research in vitro and in human liver chimeric mice. Preclinical validation of this system on serum samples from patient on or off antiviral therapy also illustrates a promising application that is rapid and cost-effective in monitoring HBV and HDV viral loads simultaneously.
Show More
| # | Filename | Filesize |
|---|---|---|
| 1 | 20240429-DATASET_Development of a dual channel detection ... | 41 KB |
| 2 | Fig 1B.csv | 352 Bytes |
| 3 | Fig 1C.csv | 362 Bytes |
| 4 | Fig 1D.csv | 464 Bytes |
| 5 | Fig 1F.csv | 506 Bytes |
| 6 | Fig 1G.csv | 823 Bytes |
| 7 | Fig 1H.csv | 732 Bytes |
| 8 | Fig 1I.csv | 435 Bytes |
| 9 | Fig 2C.csv | 126 Bytes |
| 10 | Fig 2D.csv | 155 Bytes |
| 11 | Fig 2E.csv | 445 Bytes |
| 12 | Fig 3A.csv | 952 Bytes |
| 13 | Fig 3B.csv | 634 Bytes |
| 14 | Fig 3C.csv | 536 Bytes |
| 15 | Fig 3D.csv | 443 Bytes |
| 16 | Fig 3E western blot images-repeat experiment.jpg | 548 KB |
| 17 | Fig 3E western blot images.jpg | 622 KB |
| 18 | Fig 3E.csv | 335 Bytes |
| 19 | Fig 4A.csv | 81 Bytes |
| 20 | Fig 4B and C-orginal images.jpg | 1.86 MB |
| 21 | Fig 4D.csv | 548 Bytes |
| 22 | Fig 4E.csv | 392 Bytes |
| 23 | Fig 4F.csv | 233 Bytes |
| 24 | Fig 5C.csv | 540 Bytes |
| 25 | Fig 5D.csv | 471 Bytes |
| 26 | Fig 5E.csv | 482 Bytes |
| 27 | Fig 5F.csv | 310 Bytes |
| 28 | Fig 6A.csv | 366 Bytes |
| 29 | Fig 6B.csv | 370 Bytes |
| 30 | Fig 6D.csv | 521 Bytes |
| 31 | Fig 6E.csv | 332 Bytes |
| 32 | Supplementary Fig 1D.csv | 194 Bytes |
| 33 | Supplementary Fig 2-HBV DNA.csv | 156 Bytes |
| 34 | Supplementary Fig 2-HBV DNA.jpg | 60.3 KB |
| 35 | Supplementary Fig 2-HDV RNA.csv | 157 Bytes |
| 36 | Supplementary Fig 2-HDV RNA.jpg | 66.7 KB |
| 37 | Supplementary Fig 4A.csv | 154 Bytes |
| 38 | Supplementary Fig 4B.csv | 943 Bytes |
| 39 | Supplementary Fig 4C.csv | 400 Bytes |